Duchefa haarlem the netherlands
WebOct 25, 2016 · Biotinylated proteins are visualized by staining with 5-bromo-4-chloro-3-indoyl phosphate (BCIP) and nitroblue tetrazolium (NBT) (Duchefa, Haarlem, The Netherlands) in 0.1 M Tris–HCl, pH 9.5, 5 mM MgCl 2, 0.15 mg/mL BCIP, 0.30 mg/mL NBT. To avoid background staining, we recommend to wash the membranes (twice 10 min) with TBST … WebArticle Snippet: Depending on the selection marker used, transformants were selected on Czapek Dox agar (CDA, Oxoid, Basingstoke, Hampshire, England) containing 100 μg/ml …
Duchefa haarlem the netherlands
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WebMar 10, 2024 · An aliquot of a suspension (1 ml containing ∼100 mg proliferating SEs) was spread on a sterile filter paper (Whatman No. 2, diameter 11 cm), air-dried at ambient temperature, and placed on a Petri dish in an mLV medium supplemented with 80 μM abscisic acid (ABA; Duchefa, Haarlem, The Netherlands) and 0.2 M sucrose, and … WebMar 17, 2014 · Seeds of tomato were sown on half-strength Murashige and Skoog medium with 2 g/L Gelrite Gellan Gum (Sigma-Aldrich, Milan, Italy) in covered plastic sterile vessels (Duchefa Biochemie, Haarlem, The Netherlands), and then plants were grown in the dark for 5 days at 24 °C.
WebContact Us Get in touch with us via phone or email. EMAIL [email protected] PHONE +31- (0)23-531 90 93 MAIL ADDRESS DUCHEFA FARMA B.V P.O. Box 809 2003 RV Haarlem The Netherlands VISITING ADDRESS A. Hofmanweg 71 2031 … WebNov 27, 2024 · Cells were cultivated in a liquid medium containing 4.3 g L −1 Murashige and Skoog salts (Duchefa, Haarlem, The Netherlands), 30 g L −1 sucrose, 200 mg L −1 KH 2 PO 4, 100 mg L −1 inositol, 1 mg L −1 thiamine and 0.2 mg L −1 2, 4-dichlorophenoxyacetic acid (2, 4-D), which was adjusted to a final pH of 5.8 and supplemented with 30 mg ...
WebDuchefa Farma B.V. is a supplier of pharmaceutical raw materials for small, midsize and large companies. Skip to main content LinkedIn. ... Haarlem, North Holland 534 followers WebBiochemie B.V., Haarlem, The Netherlands). The medium was autoclaved (20min at 1218C) for 2856 L. Fontanili et al. Downloaded by [University of Florida] at 09:28 24 October 2024 . sterilization. All cultureswere incubated at 25 ^ 18C ... Microbox Eco2 (Duchefa, Micropoli, Italy; 80mm £ 125mm£ 65mm, volume 650ml) with 15
WebMar 27, 2024 · All chemicals used in culture were purchased from Duchefa Biochemie (Haarlem, The Netherlands). 4.2. Tuber Induction. Single-node cuttings were conducted following a previous report . Plantlets were grown in culture tubes under long-day conditions, and then transferred to SD conditions for 6 days. Plants were then cut to one-node stem …
WebDuchefa Biochemie BV at A. HOFMANNWEG 71 2031 BH HAARLEM THE NETHERLANDS. Find their customers, contact information, and details on 11 … switzerland gdp per capita 1980WebApr 12, 2024 · At 24 h post treatment, MTT solution (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; Duchefa, Haarlem, The Netherlands) was added to the culture medium at 0.4 mg/mL. After 2 h of incubation at 37 °C, the culture medium was discarded, and 100 μL DMSO was added into each well to thoroughly dissolve MTT … switzerland gay marriageWebThe root tips were digested in a pectolytic enzyme mixture [0.1% (w/v) pectolyase Y23 (Duchefa, Haarlem, the Netherlands), 0.1% (w/v) cellulase onozuka RS (Duchefa, Haarlem, the Netherlands) and 0.1% (w/v) cytohelicase (Sigma-Aldrich, Steinheim, Germany)] in 10 mM citrate buffer (10mM tri sodium citrate 10 mM citrate, pH 4.5) at 37 … switzerland gas stationsWebDuchefa Biochemie B.V. A. Hofmanweg 71, 2031BH Haarlem - Netherlands. Want to see more results ? {0} important information hidden . Log In / Sign Up. Categories. Main … switzerland geneva live road camerasWebColistin sulphate Duchefa Biochemie Home Products Antibiotics / Selective agents Colistin sulphate C0118 Colistin sulphate CAS number 1264-72-8 Potency > 19.000 units / mg Description Technical info MSDS Certificate of Analysis A mixture of the sulphates of polypeptides produced by certain strains of Bacillus polymixa. switzerland genealogy sitesWebArticle Snippet: Radial growth tests were performed once on TMA and Gamborg agar (0.305% [w/v] Gamborg B5 basal salt mixture [Duchefa, Haarlem, The Netherlands], 10 … switzerland gdp growth 2020 septemberWebFeb 9, 2024 · The pH of the medium was adjusted to 5.7–5.8 and solidified with 0.25% (w v-1) phytagel (Duchefa, Haarlem, the Netherlands), before autoclaving at a pressure of 1.06 kg cm-2 and 121 °C for 15 min. Four explants were cultured per jar, and the cultures were incubated at 25 ± 2 °C in the darkness and sub-cultured every 3 weeks. ... switzerland gateway to hell