High-fidelity cas13
Web12 de ago. de 2024 · More information: Huawei Tong et al, High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects, Nature Biotechnology (2024). … Web23 de ago. de 2024 · 然而，Cas13 普遍存在旁切活性，会造成脱靶效应，并且还可能会存在毒副作用，这成为 Cas13 走向临床应用所面临的最大障碍。 因此，如何降低或消除 Cas13 蛋白的旁切活性，开发更特异的高保真 Cas13 蛋白对基于 RNA 编辑的基因治疗策略研发和后续临床转化具有重要意义。
High-fidelity cas13
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WebHigh-fidelity Cas13 variants show similar RNA knockdown activity to wild-type Cas13 but no detectable collateral damage in transgenic mice or adeno-associated-virus-mediated … Web29 de ago. de 2024 · Off-target editing is also a critical issue in the Cas13 system, although Cas13 is already applied to the knockdown of transcripts, editing of base RNA and live …
Web27 de jul. de 2024 · Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas13 has drawn broad interest to control gene expression and cell fate at the RNA level in general. Apart from RNA interference mediated by its endonuclease activity, the nuclease-deactivated form of Cas13 further provides a versatile RNA-guided RNA-targeting … Web8 de abr. de 2024 · PDF The TDP-43 proteinopathies, which include amyotrophic lateral sclerosis and frontotemporal dementia, are a devastating group of neurodegenerative... Find, read and cite all the research ...
Web23 de fev. de 2024 · Here, we demonstrated that RNA targeting of paternal Ube3a-ATS with a high-fidelity CRISPR-Cas13 (hfCas13x.1) system could restore Ube3a expression to … Web18 de fev. de 2024 · A high-fidelity RNA-targeting Cas13 restores paternal Ube3a expression and improves motor functions in Angelman syndrome mice. Author links open overlay panel Jinhui Li 1 6, Zhixin Shen 1 6, Yajing Liu 1 6, Zixiang Yan 3 6, Yuanhua Liu 1 6, Xiang Lin 4, Junjie Tang 1, Ruimin Lv 1, Guannan Geng 1, Zhi-Qi Xiong 1, Changyang …
Web13 de jan. de 2024 · Hence high-fidelity Cas13 variants with minimal collateral effects have been developed, which could further elevate their utility for targeted degradation of RNAs …
Web28 de set. de 2024 · CRISPR-Cas13 systems have recently been employed to degrade RNA in yeast, plants, and mammalian cell lines. However, no systematic study of the potential of Cas13 has been carried out in an animal system. Here, we show that CRISPR-RfxCas13d (CasRx) is an effective and precise system to deplete specific mRNA … lament darkherWeb1 A high-fidelity RNA-targeting Cas13 restores paternal Ube3a expression and improves motor functions .. 来源：mol ther( P 1525-0016 E 1525-0024 ) 发表时间: 2024/02. lament burn satan burnWeb17 de nov. de 2024 · Advances in CRISPR technology have immensely improved our ability to manipulate nucleic acids, and the recent discovery of the RNA-targeting endonuclease Cas13 adds even further functionality. Here, we show that Cas13 works efficiently in Drosophila, both ex vivo and in vivo. We test 44 different Cas13 variants to identify … jersey rib tripsWeb16 de ago. de 2024 · The study, published in Nature Biotechnology in the paper, “High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects,” demonstrates the feasibility of hfCas13 for efficient on-target RNA degradation with almost no collateral damage in mammalian cells and animals. Concerns have been raised over … jersey remakeWeb11 de ago. de 2024 · High-fidelity Cas13 variants show similar RNA knockdown activity to wild-type Cas13 but no detectable collateral damage in transgenic mice or adeno … jersey rnli disputeWeb12 de ago. de 2024 · Thus, high-fidelity Cas13 variants with minimal collateral effects are now available for targeted degradation of RNAs in basic research and future therapeutic … lament dydonyWebrpa虽然用的也较多，不过rpa需要最后一步将扩增产物转化为rna分子，因此，它经常与cas13酶结合使用,它最主要的优势是rpa反应的温度跟crispr反应温度接近，但是最大局限性是和crispr扩增的产物浓度较低。它在靶标浓度低的时候灵敏度不如lamp好。 jersey roadmap