WebSep 5, 2016 · The multimode separation mechanism of the OmniPac PAX-500 is achieved by coating a macroporous, hydrophobic polymer core for RP retention with an anion-exchange latex bead layer for anion-exchange retention. The macroporous structure provides an accessible hydrophobic core where reversed-phase retention occurs. WebIn the case of HILIC separations there is no ion pairing reagent needed, and the oligonucleotide retention is based on a partitioning mechanism between the mobile phase …
HILIC Retention Mechanisms Thermo Fisher Scientific - US
Web3.15.5.2.2 HILIC Hydrophilic interaction liquid chromatography (HILIC) is a separation technique in which the analytes interact with a hydrophilic stationary phase in the column. … WebHILIC retention mechanism is more complex in comparison to other chromatographic separation modes and many scientists are still striving to understand it fully. It is often described as a mixed-mode mechanism, where several kinds of interaction contribute simultaneously to the overall retention. britta naumann-knapp
A new high-throughput method based on hydrophilic interaction …
WebHILIC provides the advantage of retaining highly polar analytes 1-3 that are difficult to separate on reversedphase columns such as C18. Simple sugars are well suited for this type of chromatography due to their polar nature. Silica bonded with an aminopropyl silane is a popular phase for HILIC separation of sugars. WebAug 15, 2013 · It is also important to notice that the ion-exchange mechanism, always present with HILIC phases, plays a major role for the retention of peptides. Based on my own experience, I would indicate that 95% of peptides can be adequately analysed in HILIC thanks to the ion-exchange contribution. WebAug 1, 2013 · TSK-gel NH2 column showed mixed HILIC-ion-exchange mechanism, the highest separation efficiency and better selectivity and resolution for tested analytes than the other studied column, especially ... britta neumann