Web基因敲除及试剂盒。选定待敲除基因的编码区的至少两个目标序列,使其分别包含完整的目标密码子CAA、CAG或CGA;利用至少两个sgRNA序列来将BE3定位到相应的目标序列以使目标密码子中的目标单碱基C变成T从而相应引入终止密码子TAA或TAG、TGA以实现基因敲除;所述至少两个sgRNA序列为分别与所述至少 ... WebpMD19-T Vector is a high-efficiency TA cloning vector constructed from pUC19. The pMD19-T Vector is 2.6kb in size and contains the ampicillin resistance gene for selection. …
Addgene: th-P2A-EGFP donor
WebDNA 片段的回收一. 实验目的 1. 掌握转化的原理和方法2. 掌握 TA 克隆的原理3. 掌握 互补的原理和蓝白斑筛选重组子的原理和方法二. 实验仪器与试剂1. 实验仪器:恒温摇床超净工作台恒温水浴锅恒温箱微量取液器等2. 实验材料:连,文客久久网wenke99.com WebpMD19-T序列. pMD19-T序列_生物学_自然科学_专业资料。载体序列pMD19-T Vector Positions ... pMD19-T Simple 2页 免费 pMD-19T 7页 免费 pMD19-T序列txt版本 2页 .... pMD19-T. pMD19-T Simple 2页 免费 pMD19-T Simple 2页 免费 pMD19-T序列txt版本...pMD19-T Vector Positions of various elements Vector size (bp) Multiple .... pMD19-T … ogee cut instructions
Prokaryotic expression, purification, physicochemical properties and …
Web蔡 曼,李卫华,王 娟,王旭文,孔宪辉,余 渝,刘 丽*(1 石河子大学/新疆兵团绿洲生态农业重点实验室,新疆石河子 832003 ... WebNov 1, 2024 · The PM H +-ATPase is the most dominant membrane protein in higher plants, consisting of a single polypeptide of approx. 100 kDa in size and belongs to the P-type ATPases superfamily ( Janicka et al., 2024 ). It is located on the plasma membrane and pumps H + ions out of cells. WebApr 6, 2024 · The amplified target fragment was cloned into the pMD-19T Simple vector (TaKaRa, Dalian, China) for DH5α (TIANGEN) transformation. After colony screening and identification, positive clones were grown in a 5 mL LB culture medium, and plasmid isolation was performed using a plasmid MiniPrep Kit (Sangon Biotech). ogee edge backsplash