Psba promoter induction
WebJan 1, 2014 · In this protocol we will show how to transform, screen, and monitor … WebJul 24, 2015 · This was achieved by cloning CTB fused to Mycobacterium tuberculosis derived epitopes, i.e., the 6 KD early secretory antigenic target (ESAT-6) and Mtb72F (a protein resulting from the fusion of two TB antigens, Mtb32 and Mtb39), downstream of the psbA promoter that normally regulates expression of the protein D1, a component of the …
Psba promoter induction
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WebSep 4, 2008 · Blue‐light‐receptor mutational analysis revealed that AtSIG5 ‐specific two‐phase induction is mediated through cryptochrome 1 and cryptochrome 2 at lower fluences and more significantly through cryptochrome 1 at higher fluences. In mature chloroplasts, the promoters of psbA and psbD are predominantly recognized by AtSIG5 … WebThe chloroplast psbA promoter produces an 18.5 fold increase in expression in E. coli …
WebThe chloroplast psbA promoter has been found to be the strongest promoter for foreign … WebFeb 12, 2024 · The constructed vector with a heat-inducible promoter was then transformed into C. reinhardtii and expected properties of transformant cells were showed with a down-regulated expression of the target gene psbA against a corresponding up-regulated expression of amiRNA-D1.
WebJun 13, 2024 · Homologous targeting of the 16S- trnI / trnA -23S region and four endogenous regulatory elements, including the psbA promoter, rbcL promoter, rbcL terminator, and psbA terminator in H. pluvialis, were performed to construct a chloroplast transformation vector for … WebNov 1, 2003 · The expression of the green fluorescent protein reporter gene (gfp) from the bacterial trc and plastid rrn and psbA promoters has been compared in transplastomic tobacco plants produced by...
WebThe chloroplast psbA promoter has been found to be the strongest promoter for foreign gene expression in chloro- plasts (Daniell and McFadden, 1987; Daniell, 1993). Expression of a foreign...
WebSep 22, 2024 · Induction of a group 2 σ factor, RPOD3, by high light and the underlying mechanism in Synechococcus elongatus PCC 7942. J. Biol. Chem. 2007; 282 ... desirable in further analysis of such regulatory pathways. Nonetheless, direct binding assays of sigma factors with psbA promoter regions can provide immediate insight into their … teacher shirt websiteteachers hitting studentsWebApr 1, 2024 · The endogenous phytoene desaturase coding sequence (pds) has been codon optimized and overexpressed in the chloroplast of H. pluvialis under the control of the psbA promoter/UTR. After biolistic transformation and selection, PDS mRNA and protein were detected in transformants. teacher shirt white dressWebJan 17, 2024 · Moreover, efficient expression of uidA gene under the heterologous rice … teachers hitting on studentsWebMar 1, 2024 · Specific promoter recognition by RNA polymerase and transcription initiation relies on sigma factor proteins in prokaryotes [3., 4., 5., 6.The expression of cyanobacterial psbA genes and their light-responsive nature is regulated by the housekeeping sigma factor and alternative sigma factors respectively (Figure 1 A).Mutants of Synechocystis lacking … teachers hmu grWebWe show that fusion of the 16S promoter had little impact on protein accumulation from the psbA 5¢ UTR in a psbA-deficient genetic background. Furthermore, the 16S⁄psbA promoter⁄UTR fusion was silenced in the presence of wild-type levels of D1 protein, confirm-ing that the psbA 5¢ UTR is the primary target for D1-dependent auto-repression. teachers hmu.grWebApr 3, 2007 · It has been reported that the principal σ factor SigA can recognize the psbA promoters for basal transcription, and SigD contributes to the light-induced transcription of psbA under light in PCC 6803 cells [9], ... Nitrogen induction of sugar catabolic gene expression in Synechocystis sp. PCC 6803. DNA Res., 13 (2006), pp. 185-195. teachers hmu