WebDr. Kamoun has made outstanding contributions to the science of plant pathology that have been described in more than 50 journal articles and book chapters. His research at Ohio … WebI have been trying to do a co-IP using FLAG tagged and HA tagged proteins. Did another western after transfecting each 1XHA -A protein (55kD) and 3X FLAG-B-protein (69 Kd) …
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WebSmall peptide tag (11 amino acids) Binds to the c-Myc Monoclonal Antibody. Myc-tagged protein can be eluted with low pH buffer or sample buffer. A Myc-tag can be incorporated on either the N-terminus, the C-terminus, or internally. Can be expressed in bacterial, yeast, mammalian, and baculovirus-infected insect cells. WebThe standard FLAG ® peptide (sequence: DYKDDDDK) is a small tag that can be incorporated with minimal risk of steric hindrance or negative impact on protein solubility The 3xFLAG ® tag sequence fuses three tandem FLAG ® epitopes for enhanced (up to 200X) detection of fusion proteins FLAG ® Tag Protein Purification hardsubbing programs for vocaloid
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WebTested applications:WB IHC IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP Reactivity: Western blot analysis of 293T, using DDDDK-Tag antibody (AE063) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. ... FLAG-tag, or FLAG octapeptide, or FLAG epitope, … WebMar 3, 2024 · Sophien Kamoun group Sophien Kamoun and his Team study plant-pathogen coevolution and how it impacts mechanisms of virulence and immunity. … WebJun 29, 2024 · He found that he enjoyed the rigorous approach of molecular biology, and he began to study genetics. He combined this with his original passion of understanding how the world works by taking courses in evolution. But, staying in the lab wasn’t enough. Dr. Kamoun wanted to bridge the gap between the lab and practical applications. change local user name windows10